1.Consecutive tissue sectioning. 4 µm sections are cut sequentially and stained. A central slide is used as a reference slide, with special stains to assist in automated feature analysis, as necessary. Two to four serial sections above and below the reference slide are stained with the biomarker of interest

2.Automated feature recognition. Image analysis is run on the central reference slide to detect regions of interest, often assisted by special stains.

3.Image and ROI registration. Advanced image registration techniques transfer the features identified in the reference slides to the target slides. Image analysis can then be run on the target slides to measure individual IHC, biocompatiblity, or lesion effects.

4.QC and pathologist review. An histology technician checks the results and eliminates any poorly annotated regions. The pathologist then reviews the slides and signs off on the analysis.