In a collaboration with EPL, the leading GLP pathology peer review organization, Flagship Biosciences presented continued work at the Society of Toxicology Pathology meeting in Denver, Colorado in June 2011 on virtual peer review. The Virtual Imaging in Peer Review or VIPER was a consortium of multiple pharma companies started in 2010 to evaluate international [...]
December 31, 2010 – 11:33 pm This is another example of image analysis on dual stained slides, demonstrating the ability to quantify lymphatic tissue. The dual stain from Biocare is D2-40 (Fast Red) a new selective marker of lymphatic endothelium in normal tissues and vascular lesions that does not stain adjacent capillaries. The Ki-67 nuclear antigen is marked with DAB. Together these [...]
November 3, 2010 – 10:11 pm You wouldn’t dream of downhill skiing without a rating system. The beginners can stay off the tough slopes and the pros get to debate whether the black run of Colorado’s Arapahoe Basin Pallavicini is tougher than any double diamond at Breckenridge. We need the same rating system in image analysis. Many of the double-diamond examples [...]
November 2, 2010 – 9:21 pm There have been some exciting developments in work to expand the number of markers that can be measured in tissue. The constraints are cost and regulatory acceptance. Multiplexing of proteins in soluble samples (e.g. serum, plasma, urine) have advanced to true high-throughput, with arrays of ELISA-related technologies. However, tissue multiplexing remains difficult. Immunofluorescence can easily [...]
January 22, 2010 – 12:06 pm Industry has accepted relative scoring of toxicity lesions for many years. However, the real demand from investigators in the area of discovery toxicology is for quantitative data. In the scenario below, there are four images of periportal fibrosis in the livers of diabetic rats. The upper left, the control, clearly shows an increase in fibrosis as compared to the other [...]
January 20, 2010 – 5:00 pm There are many times when a pathologist needs to randomly sample tissue in a directed manner. This is to assure that similar tissue substructures are sampled in a manner that prevents biasing of results. If the problem was as straightforward as sampling ANY area, then random sampling algorithms would have already been developed for this. Unfortunately in real [...]
By Frank | Also posted in liver | Tagged angiogenesis, Aperio, biostatistics, digital pathology, directed random sampling, histology pattern recognition, IHC, image analysis, ImageScope, liver, toxicology | January 20, 2010 – 12:45 am The more that we use whole slide analysis, the more we are amazed that anyone tolerates the old way of doing things: doing image analysis or manual reads on small “representative” sample areas. Let’s look at a mouse spleen example. Histology pattern recognition was tested on mouse spleen sections in an effort to quantitate normal [...]
January 15, 2010 – 9:16 am Experienced pathologists are very good at using minimum magnification to look at a slide. A pathologist can see more scanning an entire slide at 5x and then occasionally jumping to 20x or 40x in a couple of areas, than starting out at 40x. We all know this, and take it for granted as pathologists. However, [...]
