Below is the video from a webinar we gave on a new algorithm for measuring vacuolar spaces. Part 1 Part 2 Part 3 Presenters: Frank Voelker Event: Aperio Webinar
We receive frequent requests for measuring brown fat. Below are some examples, with the same Flagship fat algorithm that was used previously for white fat and lung alveoli.
White fat measurement is shown below with a Flagship algorithm that first segments and then individually identifies each fat vacuole. Statistics of area and perimeter per vacuole are counted, and histograms can be output as required. Some results are shown below. In areas where vacuoles are either not easily seen by eye, or where they [...]
Measurement of hepatocellular glycogen is an appropriate exercise in determining level of gluconeogenesis in rodent efficacy studies for evaluating potential antidiabetic compounds. However, accuracy in glycogen quantitation requires strict control of the fasting period in all study animals because intracellular levels can vary greatly depending on postprandial time. Technical challenges in visualizing glycogen also exist because it is highly soluble, and [...]
Industry has accepted relative scoring of toxicity lesions for many years. However, the real demand from investigators in the area of discovery toxicology is for quantitative data. In the scenario below, there are four images of periportal fibrosis in the livers of diabetic rats. The upper left, the control, clearly shows an increase in fibrosis as compared to the other [...]
There are many times when a pathologist needs to randomly sample tissue in a directed manner. This is to assure that similar tissue substructures are sampled in a manner that prevents biasing of results. If the problem was as straightforward as sampling ANY area, then random sampling algorithms would have already been developed for this. Unfortunately in real [...]
