January 23, 2011 – 3:31 pm In this example we use multimodal scanning of a brightfield stained slide in both brightfield and fluorescence to better delineate red from white pulp in spleen. The two images are overlaid with image registration, showing fluorescence scanning is better than brightfield for a more clear differentiation of red/white pulp. This aids in running more accurate [...]
By Erik Hagendorn | Also posted in Fluorescent scanning, spleen, toxicology | Tagged fluorescence, fluorescence scanning, fluorescent, fluorescent scanning, image registration, Multimodal, pulp, spleen, whole slide scanning | January 18, 2011 – 11:51 pm New monthly webinar series covering tissue engineering, biomaterials, biocompatibility, and other topics related to the tissue-material interface.
By Steve Potts | Also posted in cardiology, cardiovascular, heart, medical devices | Tagged biomaterial, biomedical engineering, colorado state university, digital pathology, Dr. Robert Kellar, new imaging, northern arizona university, quantitative analysis, synthetic materials, tissue engineering, webinar | January 17, 2011 – 7:08 pm Quantitative dermatopathology is just scratching the surface with digital pathology
By Steve Potts | Also posted in Clinical trials regulatory aspects of digital pathology, dermatology, Image analysis in CAP and CLIA regulated laboratories, large pharma, medical devices, skin, small biotech | Tagged aarhus university, comparative pathology, dermatology, dermatopathology, digital pathology, fluorescence scanning, image analysis, Multiplexed IHC, ophthalmology, proliferation, scanning technology, university in denmark, university of pittsburg | January 8, 2011 – 4:22 pm Phosphomarkers do not generally represent any additional difficulties in image analysis, although the sample handling and collection is not trivial, and one must take care with interpretation, particularly when working in realworld clinical specimen settings. Below is a phospho-NF-kappa B p65 (Ser 276) antibody from EnVision (Rabbit) on formalin fixed paraffin embedded human colon carcinoma. [...]
By Frank | Also posted in Colorectal, large pharma, oncology, small biotech | Tagged colon cancer, colon carcinoma, EnVision, FACTS, human colon, image registration, immunohistochemistry, NF-kappa, NF-kappaB, p65, pathologist, pattern recognition, phospho, phosphomarker, Phosphomarkers, tumor cells | January 5, 2011 – 2:54 pm Bridge Staining with Multimodal Scanning. In the images below each slide has been scanned once in brightfield and once in fluorescence, using dyes with multimodal scanning compatibility. The upper image is autofluorescence, in the lower image the DAPI has a very similar staining pattern to a fluorescence compatible hemotoxylin equivalent.
January 2, 2011 – 5:34 pm Whole slide fluorescence scanning of arteries to quantitate the tunica intima.
January 2, 2011 – 5:15 pm We have a number of multi-modal whole slide scanning techniques that we call bridge scanning, where a number of fluorescent and brightfield scans are overlaid, either from a single slide or from multiple consecutive sections. In this example, we are using FACTS with other consecutive sections to identify biocompatible responses to implanted biopolymers. We can [...]
December 31, 2010 – 10:36 pm Quantification of proliferating nuclei with multiplexed IHC in between melanoma and melanocytic nevi.
By Frank | Also posted in dermatology, Multiplexed IHC, skin | Tagged Genie, histology pattern recognition, image analysis, Ki67, melanocytes, melanocytic lesions, Multiplexed IHC, proliferation, tyrosinase, Visiopharm | November 3, 2010 – 11:09 pm Lung is a notoriously difficult organ for conducting reproducible image analysis, due to both the challenges of consistent histology processes in lung tissue and the heterogeneity of various normal and neoplastic pulmonary features. Below is an example of how histology pattern recognition can be combined with both cell and area based image analysis. Pulmonary adenocarcinomas [...]
(CISH (Chromogenic In-Situ Hybridization) is a new technique first published by Tanner and his collegues (Am J Pathol 2000, 157:1467-1472). It will likely prove to be much more economical than FISH (FISH can range up to ten times the cost of IHC), and can be conducted with an ordinary microscope. Because it is chromogenic, the [...]
