New monthly webinar series covering tissue engineering, biomaterials, biocompatibility, and other topics related to the tissue-material interface.
This example came from healthy porcine aorta that was stained with Hematoxylin and Eosin (H&E). Using an area-based algorithm, we can quantitatively evaluate the sample for elastin percentage. Elastin is a critical component of the microstructure within large arteries such as the aorta. This type of quantitative analysis can be performed on various types of [...]
We receive frequent requests for measuring brown fat. Below are some examples, with the same Flagship fat algorithm that was used previously for white fat and lung alveoli.
White fat measurement is shown below with a Flagship algorithm that first segments and then individually identifies each fat vacuole. Statistics of area and perimeter per vacuole are counted, and histograms can be output as required. Some results are shown below. In areas where vacuoles are either not easily seen by eye, or where they [...]
Below is a cross section of mouse heart stained with sirius red which stains positively for fibrosis. Interwoven trabeculae of positively staining fibrous connective tissue (see insert) are visible within the ventricular myocardium as a result of experimental damage. With this mouse heart fibrosis model, it would be desirable to measure area of fibrosis as a percent of heart cross-sectional area, [...]
Histology pattern recognition could be used to identify and measure the area of necrotic core, foam cells and heart tissue versus media in rodent preclinical cardiovascular samples.
The need to identify tissues in a artery cross-section is very common in pathology review in medical devices. Below is an example using histology pattern recognition, where the computer has been trained to recognize the different layers.
A scaffold-based, three-dimensional, human dermal fibroblast culture (3DFC) was used as a cardiac patcha to stimulate revascularization and preserve left ventricular (LV) function of the infarcted LV in severe combined immunodeficient (SCID) mice. The histopathology image below illustrates the differences between the venules, arterioles, and capillaries. Image analysis is able to identify these vessels and [...]
