In a collaboration with EPL, the leading GLP pathology peer review organization, Flagship Biosciences presented continued work at the Society of Toxicology Pathology meeting in Denver, Colorado in June 2011 on virtual peer review. The Virtual Imaging in Peer Review or VIPER was a consortium of multiple pharma companies started in 2010 to evaluate international [...]
February 13, 2011 – 2:29 pm We do a lot of projects involving comparing a protein’s expression in the nucleus versus cytoplasm. Many proteins show activation upon translocation from cytoplasm to nucleus. Below are some example steps that we perform to obtain a measurement of the ratio on a cell-basis. There are a wide number of variations to these approaches. The [...]
January 23, 2011 – 3:31 pm In this example we use multimodal scanning of a brightfield stained slide in both brightfield and fluorescence to better delineate red from white pulp in spleen. The two images are overlaid with image registration, showing fluorescence scanning is better than brightfield for a more clear differentiation of red/white pulp. This aids in running more accurate [...]
By Erik Hagendorn | Also posted in Fluorescent scanning, spleen, toxicology | Tagged fluorescence, fluorescence scanning, fluorescent, fluorescent scanning, image registration, Multimodal, pulp, spleen, whole slide scanning | January 18, 2011 – 11:51 pm New monthly webinar series covering tissue engineering, biomaterials, biocompatibility, and other topics related to the tissue-material interface.
By Steve Potts | Also posted in cardiology, cardiovascular, heart, medical devices | Tagged biomaterial, biomedical engineering, colorado state university, digital pathology, Dr. Robert Kellar, new imaging, northern arizona university, quantitative analysis, synthetic materials, tissue engineering, webinar | January 17, 2011 – 7:08 pm Quantitative dermatopathology is just scratching the surface with digital pathology
By Steve Potts | Also posted in Clinical trials regulatory aspects of digital pathology, dermatology, Image analysis in CAP and CLIA regulated laboratories, large pharma, medical devices, skin, small biotech | Tagged aarhus university, comparative pathology, dermatology, dermatopathology, digital pathology, fluorescence scanning, image analysis, Multiplexed IHC, ophthalmology, proliferation, scanning technology, university in denmark, university of pittsburg | January 8, 2011 – 4:22 pm Phosphomarkers do not generally represent any additional difficulties in image analysis, although the sample handling and collection is not trivial, and one must take care with interpretation, particularly when working in realworld clinical specimen settings. Below is a phospho-NF-kappa B p65 (Ser 276) antibody from EnVision (Rabbit) on formalin fixed paraffin embedded human colon carcinoma. [...]
By Frank | Also posted in Colorectal, large pharma, oncology, small biotech | Tagged colon cancer, colon carcinoma, EnVision, FACTS, human colon, image registration, immunohistochemistry, NF-kappa, NF-kappaB, p65, pathologist, pattern recognition, phospho, phosphomarker, Phosphomarkers, tumor cells | January 4, 2011 – 11:07 pm Anti-kappa and anti-lambda detect surface light chain immunoglobulins on normal and neoplastic B-cells in human lymphoid tissue. In normal lymphoid tissue the kappa and lambda cell ratio is approximately 2:1, but values in excess of that ratio indicate monoclonality caused by either a lymphoproliferative disorder or neoplasia such as lymphoma. The double stain antibody provided by [...]
By Frank | Also posted in lymph, Multiplexed IHC, oncology | Tagged Aperio, biocare, dual staining, image analysis, immunoglobulins, kappa lambda, lymphoid tissue, lymphoproliferative disorder, Multiplexed IHC, tissue section, Visiopharm | January 2, 2011 – 5:34 pm Whole slide fluorescence scanning of arteries to quantitate the tunica intima.
January 2, 2011 – 5:15 pm We have a number of multi-modal whole slide scanning techniques that we call bridge scanning, where a number of fluorescent and brightfield scans are overlaid, either from a single slide or from multiple consecutive sections. In this example, we are using FACTS with other consecutive sections to identify biocompatible responses to implanted biopolymers. We can [...]
December 31, 2010 – 10:36 pm Quantification of proliferating nuclei with multiplexed IHC in between melanoma and melanocytic nevi.
By Frank | Also posted in dermatology, Multiplexed IHC, skin | Tagged Genie, histology pattern recognition, image analysis, Ki67, melanocytes, melanocytic lesions, Multiplexed IHC, proliferation, tyrosinase, Visiopharm | 
