March 16, 2011 – 10:29 pm The novel staining approach combines the multiplexing capabilities of fluorescence with the tissue context of brightfield whole slide scanning FLAGSTAFF, Ariz. — March 17, 2011 — Flagship Biosciences announced today a novel whole slide imaging approach called Bridge Staining™, that allows a brightfield background to be aligned and overlaid on a fluorescent image on the same [...]
By flagship | Also posted in Fluorescent scanning, NEWS | Tagged autofluorescence, brightfield, chromagen, Dr. Frank Voelker, fluorescence scanning, fluorescent, fluorochrome, immunofluorescence, multiplexing, pathology, protein expression, tissue sections, visualization, whole slide imaging, whole slide scanning | February 13, 2011 – 2:29 pm We do a lot of projects involving comparing a protein’s expression in the nucleus versus cytoplasm. Many proteins show activation upon translocation from cytoplasm to nucleus. Below are some example steps that we perform to obtain a measurement of the ratio on a cell-basis. There are a wide number of variations to these approaches. The [...]
January 4, 2011 – 11:07 pm Anti-kappa and anti-lambda detect surface light chain immunoglobulins on normal and neoplastic B-cells in human lymphoid tissue. In normal lymphoid tissue the kappa and lambda cell ratio is approximately 2:1, but values in excess of that ratio indicate monoclonality caused by either a lymphoproliferative disorder or neoplasia such as lymphoma. The double stain antibody provided by [...]
By Frank | Also posted in lymph, oncology | Tagged Aperio, biocare, dual staining, image analysis, immunoglobulins, kappa lambda, lymphoid tissue, lymphoproliferative disorder, Multiplexed IHC, tissue section, Visiopharm | December 31, 2010 – 10:36 pm Quantification of proliferating nuclei with multiplexed IHC in between melanoma and melanocytic nevi.
By Frank | Also posted in dermatology, skin | Tagged Genie, histology pattern recognition, image analysis, Ki67, melanocytes, melanocytic lesions, Multiplexed IHC, proliferation, tyrosinase, Visiopharm | November 4, 2010 – 10:37 am 1.Consecutive tissue sectioning. 4 µm sections are cut sequentially and stained. A central slide is used as a reference slide, with special stains to assist in automated feature analysis, as necessary. Two to four serial sections above and below the reference slide are stained with the biomarker of interest 2.Automated feature recognition. Image analysis is [...]
November 2, 2010 – 9:21 pm There have been some exciting developments in work to expand the number of markers that can be measured in tissue. The constraints are cost and regulatory acceptance. Multiplexing of proteins in soluble samples (e.g. serum, plasma, urine) have advanced to true high-throughput, with arrays of ELISA-related technologies. However, tissue multiplexing remains difficult. Immunofluorescence can easily [...]
