Category Archives: Fluorescent scanning

Fluorescence and brightfield in one whole slide image

The novel staining approach combines the multiplexing capabilities of fluorescence with the tissue context of brightfield whole slide scanning FLAGSTAFF, Ariz. — March 17, 2011 — Flagship Biosciences announced today a novel whole slide imaging approach called Bridge Staining™, that allows a brightfield background to be aligned and overlaid on a fluorescent image on the same [...]

White versus red pulp with fluorescence scanning

In this example we use multimodal scanning of a brightfield stained slide in both brightfield and fluorescence to better delineate red from white pulp in spleen. The two images are overlaid with image registration, showing fluorescence scanning is better than brightfield for a more clear differentiation of red/white pulp.  This aids in running more accurate [...]

Bridge Staining – Spleen and Kidney

Bridge Staining with Multimodal Scanning. In the images below each slide has been scanned once in brightfield and once in fluorescence, using dyes with multimodal scanning compatibility. The upper image is autofluorescence, in the lower image the DAPI has a very similar staining pattern to a fluorescence compatible hemotoxylin equivalent.

Fluorescence scanning of tunica intima

Whole slide fluorescence scanning of arteries to quantitate the tunica intima.