On July 12, 2011, the FDA released a new draft guidance on the development and review of companion diagnostics (CDx). I would encourage anyone working in anatomic pathology to review the draft, as it will have widespread impact on digital pathology companies, antibody providers, and anatomic pathologists in both private practices and large reference laboratories. The [...]
March 16, 2011 – 10:29 pm The novel staining approach combines the multiplexing capabilities of fluorescence with the tissue context of brightfield whole slide scanning FLAGSTAFF, Ariz. — March 17, 2011 — Flagship Biosciences announced today a novel whole slide imaging approach called Bridge Staining™, that allows a brightfield background to be aligned and overlaid on a fluorescent image on the same [...]
By flagship | Also posted in Fluorescent scanning, Multiplexed IHC, NEWS | Tagged autofluorescence, brightfield, chromagen, Dr. Frank Voelker, fluorescence scanning, fluorescent, fluorochrome, immunofluorescence, multiplexing, pathology, protein expression, tissue sections, visualization, whole slide imaging, whole slide scanning | February 13, 2011 – 2:29 pm We do a lot of projects involving comparing a protein’s expression in the nucleus versus cytoplasm. Many proteins show activation upon translocation from cytoplasm to nucleus. Below are some example steps that we perform to obtain a measurement of the ratio on a cell-basis. There are a wide number of variations to these approaches. The [...]
January 31, 2011 – 11:35 am The fifth annual retreat kicked off on Monday in sunnny Miami Florida in a packed room of 120 pathologists. Every year this conference provides the most in-depth analysis and discussion of best practices in immunohistochemistry. Below are some notes on the first day: Conference started with a pretest of 20 questions, presenting various cases and [...]
January 23, 2011 – 3:31 pm In this example we use multimodal scanning of a brightfield stained slide in both brightfield and fluorescence to better delineate red from white pulp in spleen. The two images are overlaid with image registration, showing fluorescence scanning is better than brightfield for a more clear differentiation of red/white pulp. This aids in running more accurate [...]
By Erik Hagendorn | Also posted in Fluorescent scanning, spleen, toxicology | Tagged fluorescence, fluorescence scanning, fluorescent, fluorescent scanning, image registration, Multimodal, pulp, spleen, whole slide scanning | January 5, 2011 – 2:54 pm Bridge Staining with Multimodal Scanning. In the images below each slide has been scanned once in brightfield and once in fluorescence, using dyes with multimodal scanning compatibility. The upper image is autofluorescence, in the lower image the DAPI has a very similar staining pattern to a fluorescence compatible hemotoxylin equivalent.
January 4, 2011 – 11:07 pm Anti-kappa and anti-lambda detect surface light chain immunoglobulins on normal and neoplastic B-cells in human lymphoid tissue. In normal lymphoid tissue the kappa and lambda cell ratio is approximately 2:1, but values in excess of that ratio indicate monoclonality caused by either a lymphoproliferative disorder or neoplasia such as lymphoma. The double stain antibody provided by [...]
By Frank | Also posted in lymph, Multiplexed IHC, oncology | Tagged Aperio, biocare, dual staining, image analysis, immunoglobulins, kappa lambda, lymphoid tissue, lymphoproliferative disorder, Multiplexed IHC, tissue section, Visiopharm | January 2, 2011 – 5:34 pm Whole slide fluorescence scanning of arteries to quantitate the tunica intima.
December 31, 2010 – 10:36 pm Quantification of proliferating nuclei with multiplexed IHC in between melanoma and melanocytic nevi.
By Frank | Also posted in dermatology, Multiplexed IHC, skin | Tagged Genie, histology pattern recognition, image analysis, Ki67, melanocytes, melanocytic lesions, Multiplexed IHC, proliferation, tyrosinase, Visiopharm | December 29, 2010 – 10:37 pm New approach combining laser capture micro-dissection with digital pathology and histology pattern recognition
By Steve Potts | Also posted in 2E=2L=2S Pathology Adoption, laser-capture microdissection | Tagged amyloidosis, biopsy specimen, digital pathology, epitope, histology pattern recognition, mass spec, mayo clinic, microdissection, multiplexing, post translational modifications, protein expression | 
