Category Archives: FACTS

Special stains, feature recognition, and alignment techniques on consecutive tissue sections

FACTS process

1.Consecutive tissue sectioning. 4 µm sections are cut sequentially and stained. A central slide is used as a reference slide, with special stains to assist in automated feature analysis, as necessary. Two to four serial sections above and below the reference slide are stained with the biomarker of interest 2.Automated feature recognition. Image analysis is [...]

Multiplexing protein expression in tissue

There have been some exciting developments in work to expand the number of markers that can be measured in tissue. The constraints are cost and regulatory acceptance. Multiplexing of proteins in soluble samples (e.g. serum, plasma, urine) have advanced to true high-throughput, with arrays of ELISA-related technologies. However, tissue multiplexing remains difficult. Immunofluorescence can easily [...]