In a collaboration with EPL, the leading GLP pathology peer review organization, Flagship Biosciences presented continued work at the Society of Toxicology Pathology meeting in Denver, Colorado in June 2011 on virtual peer review. The Virtual Imaging in Peer Review or VIPER was a consortium of multiple pharma companies started in 2010 to evaluate international [...]
February 13, 2011 – 2:29 pm We do a lot of projects involving comparing a protein’s expression in the nucleus versus cytoplasm. Many proteins show activation upon translocation from cytoplasm to nucleus. Below are some example steps that we perform to obtain a measurement of the ratio on a cell-basis. There are a wide number of variations to these approaches. The [...]
January 17, 2011 – 7:08 pm Quantitative dermatopathology is just scratching the surface with digital pathology
By Steve Potts | Also posted in Clinical trials regulatory aspects of digital pathology, dermatology, Image analysis in CAP and CLIA regulated laboratories, large pharma, medical devices, skin | Tagged aarhus university, comparative pathology, dermatology, dermatopathology, digital pathology, fluorescence scanning, image analysis, Multiplexed IHC, ophthalmology, proliferation, scanning technology, university in denmark, university of pittsburg | January 8, 2011 – 4:22 pm Phosphomarkers do not generally represent any additional difficulties in image analysis, although the sample handling and collection is not trivial, and one must take care with interpretation, particularly when working in realworld clinical specimen settings. Below is a phospho-NF-kappa B p65 (Ser 276) antibody from EnVision (Rabbit) on formalin fixed paraffin embedded human colon carcinoma. [...]
By Frank | Also posted in Colorectal, large pharma, oncology | Tagged colon cancer, colon carcinoma, EnVision, FACTS, human colon, image registration, immunohistochemistry, NF-kappa, NF-kappaB, p65, pathologist, pattern recognition, phospho, phosphomarker, Phosphomarkers, tumor cells | November 3, 2010 – 11:09 pm Lung is a notoriously difficult organ for conducting reproducible image analysis, due to both the challenges of consistent histology processes in lung tissue and the heterogeneity of various normal and neoplastic pulmonary features. Below is an example of how histology pattern recognition can be combined with both cell and area based image analysis. Pulmonary adenocarcinomas [...]
(CISH (Chromogenic In-Situ Hybridization) is a new technique first published by Tanner and his collegues (Am J Pathol 2000, 157:1467-1472). It will likely prove to be much more economical than FISH (FISH can range up to ten times the cost of IHC), and can be conducted with an ordinary microscope. Because it is chromogenic, the [...]
The eye serves as an important organ for toxicology research. Below is a rodent H&E ocular cross-section, followed by pathologist-trained pattern recognition of each layer. This provides a larger and faster region of interest drawing than would be used with manual ROI drawing by a technician or pathologist. Depending on the performance, Flagship will use pattern [...]
February 22, 2010 – 7:54 am We receive frequent requests for measuring brown fat. Below are some examples, with the same Flagship fat algorithm that was used previously for white fat and lung alveoli.
February 22, 2010 – 7:37 am White fat measurement is shown below with a Flagship algorithm that first segments and then individually identifies each fat vacuole. Statistics of area and perimeter per vacuole are counted, and histograms can be output as required. Some results are shown below. In areas where vacuoles are either not easily seen by eye, or where they [...]
By Trevor | Also posted in cardiology, dermatology, fat measurement, large pharma, liver, lung, medical devices, toxicology | Tagged digital pathology, fat, image analysis, object quantification, white fat | January 22, 2010 – 12:29 pm Measurement of hepatocellular glycogen is an appropriate exercise in determining level of gluconeogenesis in rodent efficacy studies for evaluating potential antidiabetic compounds. However, accuracy in glycogen quantitation requires strict control of the fasting period in all study animals because intracellular levels can vary greatly depending on postprandial time. Technical challenges in visualizing glycogen also exist because it is highly soluble, and [...]
